| J Occup Health | year | 2006 | volume | 48 | number | 2 | page | 124 | - | 128 |
| Classification | Original | |||||||||
| Title | Expression of Heme Oxygenase-1 in the Lungs of Rats Exposed to Crystalline Silica | |||||||||
| Author | Hiroko NAGATOMO, Yasuo MORIMOTO, Takako OYABU, Masami HIROHASHI, Akira OGAMI, Hiroshi YAMATO, Kaori KURODA, Toshiaki HIGASHI and Isamu TANAKA | |||||||||
| Organization | Department of Occupational Pneumology, Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health, Japan | |||||||||
| Keywords | Crystalline silica, Heme oxygenase-1, Oxidative stress, Pulmonary fibrosis | |||||||||
| Correspondence | H. Nagatomo, Department of Occupational Pneumology,
Institute of Industrial and Ecological Sciences, University of
Occupational and Environmental Health 1-1 Iseigaoka, Yahatanishi,
Kitakyushu 807-8555, Japan (e-mail: nhiroko@med.uoeh-u.ac.jp) |
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| Abstract | Expression of Heme Oxygenase-1 in the Lungs of Rats Exposed to Crystalline Silica: Hiroko NAGATOMO, et al. Department of Occupational Pneumology, Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health-Oxidative stress is thought to be the pathogenesis of pulmonary fibrosis induced by particles, and heme oxygenase-1 (HO-1) protects lung tissue against oxidative stress. We hypothesized that HO-1 is also associated with oxidative lung injury caused by exposure to particles. The present study was conducted to investigate the time course of HO-1 expression of lungs exposed to crystalline silica in vivo. Male Wistar rats were administered 1 mg or 2 mg of crystalline silica suspended in saline by a single intratracheal instillation and were sacrificed at 3 d, 1 wk, 1 month, 3 months and 6 months of recovery time. The expression of HO-1 was observed by western blot analysis and immunostaining. Protein levels of HO-1 were increased compared to the controls at 3 d, and from 1 month to 6 months following intratracheal instillation of 2 mg of crystalline silica. The levels of HO-1 were increased compared to the controls from 1 month to 6 months following intratracheal instillation of 1 mg of crystalline silica. Many HO-1 positive cells were found particularly in the alveolar macrophages during immunostaining. These findings suggest that HO-1 is related to lung injury arising from exposure to crystalline silica. | |||||||||