| J Occup Health |
year |
2001 |
volume |
43 |
number |
1 |
page |
39 |
- |
45 |
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| Classification |
Original |
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| Title |
Cytokine Profiles in Airways of Rats Exposed
to Toluene Diisocyanate |
|
| Author |
Kui-Cheng ZHENG1, Makoto ARIIZUMI1, Hidemi TODORIKI1
and Dong-Xiao NONG2 |
|
| Organization |
1Department of Preventive Medicine, 2Department
of Otolaryngology, School of Medicine, University of the Ryukyus |
|
| Keywords |
TDI, Rat, Asthma, BAL, Airway, Cytokine, Eosinophil |
|
| Correspondence |
K.-C. Zheng, Department of Preventive Medicine,
School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara,
Okinawa 903-0215, Japan |
|
| Abstract |
Cytokine Profiles in Airways of Rats Exposed
to Toluene Diisocyanate: Kui-Cheng ZHENG, et al. Department of
Preventive Medicine, School of Medicine, University of the Ryukyus-To
investigate the status of cytokines in asthmatic airways induced
by toluene diisocyanate (TDI), we established a Wistar rat model
for asthma, and did a quantitative determination of cytokine
production in asthmatic rats induced by TDI. Rats were sensitized
with two courses of intranasal application of 10% TDI with each
course for seven consecutive days, and then provoked with 5%
TDI, and were followed by observation for airway symptoms. Bronchoalveolar
lavage (BAL) was carried out, and total and differential leucocytes
from BAL fluid were counted. Lungs were histopathologically examined
for the status of inflammation in the airways. The selected cytokines,
IL-2, IL-4, IL-6 and IFN- productions, in BAL fluid in vivo
and from BAL cells cultured in vitro were quantified with ELISA
kits. The results showed that the rats with TDI sensitization
exhibited asthmatic symptoms. TDI exposure resulted in an increase
in the total number of cells, particularly eosinophils and neutrophils
in BAL fluid. Histopathological examination showed a marked infiltration
of central and peripheral airways with eosinophils in TDI-exposed
rats. In TDI-sensitized rats, the productions of IL-4 and IL-6
in BAL fluid in vivo were markedly increased compared to that
in control rats, but production of IL-2 and IFN- was similar
in the two groups. The cytokines from BAL cells cultured in vitro
were released in a similar pattern as in vivo. These findings
indicate that IL-4 and IL-6 are predominantly secreted in the
airways and may play an important role in the pathogenesis of
TDI-induced occupational allergic asthma. |