| J Occup Health |
year |
1998 |
volume |
40 |
number |
4 |
page |
302 |
- |
306 |
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| Classification |
Original |
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| Title |
Increases in DNA and Protein Syntheses in Human
Umbilical Vein Endothelial Cells Treated with Asbestos |
|
| Author |
Yuji KARASAKI1, Hajime URANO2, Akira SHIRAHATA2,
Ziro NAMBU1, Taro OHJI3, Masayoshi MIURA4 and Sadao GOTOH4 |
|
| Organization |
1School of Health Sciences, 2Department of Pediatrics
and 4Biochemistry, School of Medicine, University of Occupational
and Environmental Health and 3St. Lucia Hospital |
|
| Keywords |
Asbestos, Human umbilical vein endothelial cell,
DNA and protein syntheses |
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| Correspondence |
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| Abstract |
Increase in DNA and Protein Syntheses in Human
Umbilical Vein Endothelial Cells Treated with Asbestos: Yuji
KARASAKI, et al. School of Health Sciences, University of Occupational
and Environmental Health-When human umbilical vein endothelial
cells (HUVECs) were treated with crocidolite asbestos, HUVECs
phagocytized the fibers; no cytotoxic effect was observed when
treated at concentrations lower than 10  g/ml for 24 h, 48 h and 72 h. The
time-dependent effect of crocidolite asbestos on DNA synthesis
and protein synthesis in HUVECs was studied. The DNA synthesis
of the cells exhibited the maximum augmentation (150%) at 24
h, and the protein synthesis
increased to the maximal value (140%) at 36 h. The dose-dependent
effect of crocidolite asbestos on DNA synthesis and protein synthesis
in HUVECs was also studied. The maximum increases in DNA synthesis
and protein synthesis were observed at 5 g/ml and at 10 g/ml of the fibers, respectively.
An apparent increase in DNA synthesis may be mainly due to the
repair of DNA damage induced by asbestos under our present experimental
conditions, but also in part due to the mitogenic effect of asbestos.
Cell injury and DNA damage will remain for a while in the cells,
and asbestos fibers phagocytized into cells may induce not only
specific but also nonspecific gene transcriptions, which might
promote mutations in HUVECs and asbestosis. |